Expression and significance of miR-194 in blood of patients with gastric cancer

doi:10.3969/j.issn.1002-7386.2018.07.001

Abstract：Objective To investigate the expression and significance of miR-194 in the blood of patients with gastric cancer.Methods One hundred and twenty patients with gastric cancer who were treated in our hospital from February 2015 to July 2016 were enrolled in the study as observation group, moreover, the other 40 healthy subjects who received physical examination in our hospital at the same period were enrolled as control group.The expression levels of miR-194 were detected by RT-PCR. In addition the correlation between the expression levels of miR-194 and clinicopathological features were analyzed. Besides the serum levels of CA199,CA724 and CEA were detected by electrochemical luminescence assay. And the expression levels of BMI-1 were detected by ELISA.The correlation between the expression levels of miR-194 and the levels of BMI-1,CA199,CA724,CEA was analyzed,respectively.Results The expression levels of miR-194 in observation group were significantly lower than those in control group(P<0.05),however,the levels of CA199,CA724,CEA and target gene-BMI-1 were significantly higher than those in control group (P<0.05).Moreover the expression levels of miR-194 were not related with patient's sex and age as well as tumor size(P>0.05),however,which were correlated with the differentiation degree of tumors,TNM staging,lymphatic metastasis (P<0.05 or P<0.01).Besides the expression levels of miR-194 were negatively correlated with those of BMI-1 (r=-0.91,P<0.01),which were also negatively correlated with those of CA199,CA724, CEA (r=-0.82,P<0.05;r=-0.94,P<0.01;r=-0.72,P<0.05).Conclusion The miR-194 can be regarded as a potential biomarker in the diagnosis of gastric cancer,which may be involved in the pathogenesis and development of gastric cancer through regulating BMI-1,and it is closely related with the differentiation degree,TNM staging and lymphatic metastasis.