Effects of cryoablation combined with sorafenib and interleukin-2 on cytokines in peripheral blood of patients with advanced metastatic renal cell carcinoma

doi:10.3969/j.issn.1002-7386.2018.06.013

Abstract：Objective To observe the effects of cryoablation combined with sorafenib and interleukin-2 on cytokines in peripheral blood of patients with advanced metastatic renal cell carcinoma.Methods A total of 90 patients with advanced metastatic renal cell carcinoma were randomly divided into observation group ( n =45) and control group ( n =45).The patients in control group were treated by oral sorafenib tosylate tablets and intravenous drip with recombinant human interleukin-2,however,the patients in observation group were treated by cryoablation under local anesthesia at 2 weeks before being treated with oral sorafenib tosylate tablets and intravenous drip with recombinant human interleukin-2,with a treatment course of 4 weeks for both groups. The expression level of IFN-γ,TNF-α,IL-4,IL-6 mRNA and proteins before and after treatment were detected by Real time-PCR and Western-blot. Moreover the serum levels of IFN-γ,TNF-α,IL-4,IL-6 and ratio of IFN-γ/IL-4 before and after treatment were detected by ELISA and compared between two groups.Results There were no significant differences in the serum levels of IFN-γ,TNF-α,IL-4,IL-6 mRNA and proteins as well as ratio of IFN-γ/IL-4 before and after treatment in control group before and after treatment ( P >0.05).As compared with those before treatment, the serum levels of IFN-γ,TNF-α MRNA and proteins and IFN-γ/IL-4 ratio observation group were significantly increased after treatment,however,the levels of IL6 MRNA and proteins were significantly decreased( P <0.05). However there were no significant differences in the levels IL-4 MRNA and proteins in observation group before and after treatment( P >0.05). Conclusion The cryoablation combined with sorafenib and interleukin2 in treatment of advanced metastatic renal cell carcinoma can significantly increase the levels of IFN-γ,TNF-α MRNA and proteins and IFN-γ/IL-4 ratio so as to improve immune function of patients.