Effects of human umbilical cord mesenchymal stem cells conditioned medium on the apoptosis of hippocampus neuron induced by amyloid β in cultured rat hippocampus neuron in vitro
Abstract：Objective To investigate the effects of human umbilical cord mesenchymal stem cells conditioned medium (hUCMSCsCM) on the apoptosis of hippocampus neuron induced by amyloid β (Aβ) in cultured rat hippocampus neuron in vitro. Methods The neurons cultured for 7 ~ 8d were treated with Aβ and/or hUCMSCsCM at different concentrations,respectively.The experiment was divided into four groups: solvent control group, Aβ injury group, low dose Aβ + hUCMSCsCM group and median dose Aβ +hUCMSCsCM. The neuron viability was determined by MTT assay.The neuron apoptosis was detected by DAPI staining. The levels of cyt-c protein were measured by Western Blot. Results As compared with solvent control group,Aβ could decrease the neuron viability of primary generation hippocampus neuron in a dose and time-dependent manner ( P <0.05). As compared with Aβ injury group,hUCMSCsCM could alleviate the increase of cell vitality caused by Aβ( P <0.05). As compared with solvent control group,Aβ with final concentration of 10μmol/L for 12h increased obviously the apoptosis of primary culture hippocampus neuron. As compared with Aβ injury group,and hUCMSCsCM could decrease neuron apoptosis induced by Aβ,which could inhibit cyt-c release caused by Aβin a dose-dependent manner ( P <0.05). Conclusion The human umbilical cord mesenchymal stem cells conditioned medium can inhibit the apoptosis of primary culture hippocampus neuron caused by Aβ,which has neuroprotective effects,and its actin mechanism may be correlated with inhibiting the release of cyt-c in vitro.
Keywords：human umbilical cord mesenchymal stem cells conditioned mediumneuronapoptosisamyloid β
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 805-809 )