Prophylactic effects of non-T cell binding peptide-FNS007 on type 2 collagen-induced arthritis in rats

doi:10.3969/j.issn.1002-7386.2018.05.001

Abstract：Objective To investigate the pro phylactic effects and action mechanism of non-T cell binding peptide (FNS007) on type 2 collagen-induced arthritis in rats. Methods The CIA models of Lewis rats were established by intradermal injecting bovine type-Ⅱ collagen with incomplete Freund's adjuvant. The rats were randomly divided into six groups:blank control group, model group, methotrexate (MTX) group,FNS007 low dose group (0.25mg/kg), FNS007 middle dose group (0.5mg/kg) and FNS007 high dose group(1.0mg/kg),with 10 rats in each group. The rats in FNS007 low dose,middle dose and high dose groups were injected with different dose FNS007 respectively,at the same time of induction of CIA,once a week for 5 weeks. The rats in MTX group were injected with methotrexate and,twice a week for 5 weeks. The body weight, hind-paw swelling, arthritis scores,and histologic analysis were evaluated,moreover, the serum levels of cytokines were detected by enzyme-linked immunosorbent assays (ELISA) and histopathologic examination was performed.Results As compared with those in model group,the body weight was significantly increased in three doses FNS007 group (P<0.05).Moreover the radiographic scores in three FNS007 groups and MTX group were significantly lower than those in modeel group (P <0.05). Histopathological examination showed that FNS007 could obviously relieve the inflammation,pannus formation and damage of cartilage.In addition the serum levels of TNF-αand IL-1β in model group were significantly higher than those in blank control group(P<0.05). Furthermore FNS007 could significantly decrease the serum levels of TNF-α and IL-1β of rats with CIA (P <0. 05). Conclusion The prophylactic administration of FNS007 can significantly relieve symptoms of CIA in rats,and its action mechanism may be correlated with inhibiting T-cell activation.