Effects of bisphenol A on the expressions of P450scc and 3β-HSD protein as well as testosterone content in testis of adult SD rats
Abstract：Objective To observe the effects of bisphenol A (BPA) on the content of testosterone in testis of male rats and on the expressions of P450scc and 3βhydroxysteroid dehydrogenase(3β-HSD),and to explore its action mechanism. Methods Forty-eight adult male SD rats were randomly divided into 4 groups:control group,low dose group(2mg·kg-1·d-1), middle dose group (20mg·kg-1·d-1) and high dose group (200mg·kg-1·d-1),with continuous intragastric administration for 8 weeks. The serum levels of testosterone were measured in different dose groups,moreover the expression levels of P450scc and 3β-HSD protein in testicular interstitial cell were detected by immunohistochemistry. Results The testosterone levels in middle dose group and high dose group were significantly lower than those in control group(P<0.05), meanwhile,the testosterone levels were gradually decreased with the increasing of the BPA dose. The immunohistochemical image analysis showed that the average absorbance values of P450scc and 3β-HSD in the three different dose groups were obviously lower than those in control group,moreover,which in middle dose group and high dose group were significantly lower than those in control group (P <0.05). Conclusion The bisphenol A can inhibit the synthesis of testosterone and the expressions of the P450scc and 3β-HSD in testicular interstitial cells of SD rats.
Keywords：bisphenol Atestosterone3β-hydroxysteroid dehydrogenaseP450scc
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 531-534,538 )