The neuroprotective effects and effects of ganglioside on NSE, S100B protein and MBP levels in cerebrospinal fluid of patients with diffuse axonal injury

doi:10.3969/j.issn.1002-7386.2018.03.003

Abstract：Objective To investigate the neuroprotective effects and effects of ganglioside on neuron specificity enolization enzyme E (NSE),S100B protein and myelin basic protein (MBP) levels in cerebrospinal fluid of patients with diffuse axonal injury.Methods One hundred patients with diffuse axonal injury who were treated in our hospital from December 2013 to December 2016 were randomly divided into combination treatment group,and conventional treatment group,with 50 patients in each group.The patients in conventional treatment groups were treated by conventional hormone,dehydration to decrease intracranial pressure,stopping bleeding,maintaining balance of water and electrolyte,trophic nerve,nutrition support,fluid replacement and 0.25MPa hyperbaric oxygen within 48h after injury,however,the patients in combination treatment group,on the basis of conventional treatment group,were given ganglioside 40 ～ 80mg by intravenous drip,once a day,then these patients were followed up for 6 months,the patient's nerve function was evaluated by using U.S.national institutes of health stroke scale (NIHSS) scores,and the levels of NSE,S100B,MBP in cerebrospinal fluid were detected by enzyme-linked immunosorbent assay (ELISA),and patient's life quality was evaluated by daily life ability scale (ADL),moreover,the therapeutic effects,neurological function,wakefulness,complications,death rates and the changes of NSE,S100B,MBP levels before and after treatment as well as patient's life quality on 1 m,3m,6m after treatment were statistical analyzed and compared between two groups.Results After treatment the NIHSS scores in combination treatment group were significantly lower than those in conventional treatment group,moreover,the excellence rates in combination treatment group were significantly higher than those in conventional treatment group (P ＜ 0.05).Moreover the levels of NSE,S100B,MBP in combination treatment group were significantly lower than those in conventional treatment group (P ＜ 0.05).The wakefulness rates in combination treatment group were significantly higher than those in conventional treatment group,however,the average wakefulness time and death rates in combination treatment group were significantly lower than those in conventional treatment group (P ＜ 0.05).There were no significant differences in incidence rates of complications between two groups (P ＞0.05).Besides the ADL scores on 3m,6m after treatment in combination treatment group were significantly higher than those in conventional treatment group (P ＜ 0.05).Conclusion The ganglioside can effectively improve the therapeutic effects on diffuse axonal injury and has nerve protective effects,which can effectively promote patient's wakefulness and recovery of neural function,which can also improve the life quality of patients,and its action mechanism may be related with the improvement of NSE,S100B,MBP levels in brain,without increasing the risks of complications,therefor,which is worth using widely in clinical practice.