Experimental study on the protective effects of bone marrow mesenchymal stem cell transplantation on ovarian injury caused by chemotherapy with cyclophosphamide in vitro

doi:10.3969/j.issn.1002-7386.2018.02.016

Abstract：Objective To investigate the protective effects of bone marrow mesenchymal stem cell transplantation on ovarian injury caused by cyclophosphamide chemotherapy in vitro.Methods The rats were divided into blank group (NG group),ovariectomized group (DG group) and ovarian injury/bone marrow stem cell transplantation group (D/T group).The ovarian function indexes including estrous cycle,the expression levels of sex hormones and anti-Mullerian hormone (AMH),the number of follicles and ovary histological changes were observed and compared among the three groups.Results During the experiment,the vaginal smear in NG group showed normal estrous cycle.The oestrous cycle in DG group and D/T group was disordered after treated by cyclophosphamide.The complete estrous cycle (6 ～8 d) began to appear on 1 month after cell transplantion in 25％ (4/16) of D/T group,and complete estrous cycle appeared gradually in the other rats with time going on.The rats in DG group on 3 months after cell transplantion,the estrous cycle was still not recovered.Before modeling there were no significant differences in the levels of estradiol,follicle stimulating hormone (FSH),AMH and follicle count among the three groups (P ＞ 0.05),however,after transplantation,the levels of estradiol in DG group were significantly decreased (P ＜ 0.05),and the levels of FSH were increased gradually,moreover,which were significantly higher than those in NG group (P ＜ 0.05).The levels of estradiol in D/T group were significantly lower than those in NG group (P ＜ 0.05) on 15 days and 1 month after transplantation,and the levels of FSH were significantly higher than those in NG group (P ＜ 0.05).The expression levels of estradiol in D/T group were increased gradually on 2 months and 3 months after transplantation,however,there were no significant differences in the expression levels of estradiol and FSH between D/T group and NG group (P ＞ 0.05).Moreover the levels of AMH in DG group in every time point after transplantation were significantly lower than those in NG group (P ＜0.05),and the numbers of follicle in DG group and D/T group were significantly lower than those in NG group (P ＜ 0.05).However the numbers of follicles in D/T group were significantly higher than those in DG group (P ＜ 0.05).On 15d,1 m and 2m after transplantation,the expression levels of AMH in D/T group were significantly lower than those in NG group (P ＜ 0.05),but there were no significant differences in the expression levels of AMH on 3 months after transplantation between D/T group and NG group (P ＞ 0.05).The observation of ovarian morphology showed that the follicle in D/T group gradually returned to normal state.Conclusion The bone marrow mesenchymal stem cell transplantation can effectively repair the ovarian injury caused by cyclophosphamide chemotherapy.