Clinical significance of detection of MIF,IL-23 ,RANKL,OPG and DKK1 in peripheral blood of patients with active ankylosing spondylitis

doi:10.3969/j.issn.1002-7386.2018.02.004

Abstract：Objective To investigate the clinical significance of detection of macrophage migration inhibitory factor (MIF),interleukin-23 (IL-23),receptor activator of nuclear factor kappa B ligand (RANKL),osteoprotegerin (OPG),Dickkopf-1 (DKK1) in peripheral blood of patients with active ankylosing spondylitis (AS).Methods Forty-six patients with AS who were admitted and treated in our hospital from February 2013 to January 2016 were enrolled as observation group,at the same time,the other 38 healthy subjects were enrolled as control group.The blood specimens were collected in patients of both groups,then the levels of MIF,IL-23,RANKL,OPG,DKK1,ESR and CRP in peripheral blood of patients were detected,moreover,the correlation between the levels of these indexes and disease activity was analyzed.Results The levels of MIF (25.38 ± 11.05) ng/ml,IL-23 (374.90 + 38.19) pg/L,RANKL (21.53 ± 17.44) pg/ml,OPG (5.04 ± 1.65) pg/ml,DKK1 (0.71 ± 0.16)ng/ml in observation group were significantly higher than those in control group (4.02 ± 0.83)ng/ml,(179.82 ± 21.76) pg/L.(7.62 ± 1.24) pg/ml,(1.89 ± 0.32) pg/ml,(0.13 ± 0.04) ng/ml,respctively (P ＜ 0.01).Moreover the levels of ESR (40.25 ± 29.79) mm/h and CRP (4.78 ± 3.40) mg/L in observation group were also significantly higher than those in control group (8.27 ± 0.49) mm/h,(2.61 ± 0.35) mg/L,respectively (P ＜ 0.01).The correlation analysis results showed that the levels of MIF,OPG,IL-23 in observation group were positively correlated to those of ESR,CRP (P ＜ 0.05),however,the levels of RANKL and DKK1 were not correlated with those ESR and CRP (P ＞ 0.05).Conclusion The levels of MIF,IL-23,RANKL,OPG,DKK1 in peripheral blood of patients with active ankylosing spondylitis are obviously increased,moreover,the increase degree of MIF,IL-23,RANKL is positively correlated to disease activity,thereby,which can evaluate the severity of disease and therapeutic effects objectively.