Effects of β-acceptor blocker-bisoprolol combined with Dahuang on blood pressure, NO, eNOS and ET in experimental rats

doi:10.3969/j.issn.1002-7386.2018.01.001

Abstract：Objective To investigate the effects of bisoprolol combined with Dahuang on the changes of circadian rhythm of blood pressure (BP),the levels of nitric oxide (NO),nitric-oxide synthase (eNOS) and endothelin (ET) in experimental rats.Methods A total of 120 healthy male SD rats weighted about 250 grams were randomly divided into three groups,control group (group Ⅰ),group Ⅱ and Dahuang group (group Ⅲ),moreover,each group was randomly redivided into 4 subgroups.The rats in groupIwere given placebo by gavage,and the rats in group Ⅱ were given bisoprolol 3mg · kg-1 · d-1 by gavage,however,the rats in group Ⅲ,on the basis of group Ⅱ,were given Dahuang 300mg · kg-1 · d-1.On 1 day before medication,the levels of systolic pressure in different time pionts (02:00,08:00,14:00,20:00) were detected by rat-tail manometry,respectively in one subgroup of every group.After 4-week feeding,the levels of systolic pressure were detected by rat-tail manometry,respectively in one subgroup of every group,then all the rats were sacrificed,and the serum levels of ET-1,eNOS,NO were detected by radioimmunity method,colorimetric method and nitric acid reduction method,respectively.Results As compared with those in group Ⅰ,the levels of systolic pressure and ET-1 were decreased in group Ⅱ and group Ⅲ,however,the serum levels of NO and eNOS were increased in the two groups,moreover,the degree of increase or decrease in group Ⅲ was more obvious than that in group Ⅱ (P ＜ 0.05).In addition the circadian rhythm characteristics of blood pressure and endothelial system were significantly changed in group Ⅱ and group Ⅲ,moreover,the changes in group Ⅲ were more obvious than those in group Ⅱ (P ＜ 0.05).Conclusion The bisoprolol combined with Dahuang has synergistic effects on the serum levels of NO,eNOS and ET,which can decrease obviously blood pressure,keep circadian rhythm of blood pressure and improve endothelial system function,which suggests that combined treatment of traditional Chinese medicine and western medicine can more effectively control blood pressure.