Significance of differential expression of PTEN and Survivin in human renal clear cell cancer

doi:10.3969/j.issn.1002-7386.2017.24.002

Abstract：Objective To investigate the differences of expressions of PTEN and Survivin in human renal clear cell cancer and tumor adjacent tissues,and to explore their application value in gene-targeted therapy and surgery.Methods The expression level of PTEN and Survivin protein in human renal clear cell cancer tissues,tumor adjacent tissues (0.5cm) and adjacent tissues (1.0cm) were detected by Western Blot,moreover,the differences of expressions of PTEN and Survivin were observed,and the correlation of expressions between PTEN and Survivin was analyzed.Results The expression levels of PTEN protein in cancer tissues were significant lower than those in adjacent tissues of cancer (0.5cm) (1.482 ± 0.320 vs 10.861 ±1.347,P ＜ 0.05) and lower than those in adjacent tissues of cancer (1.0cm) (1.482 ± 0.320 vs 10.947 ± 1.744,P ＜0.05).However there were no significant differences in the expression levels of PTEN protein between adjacent tissues of cancer (0.5cm) and adjacent tissues of cancer (1.0cm) (P ＞ 0.05).The expression levels of Survivin in cancer tissues were significant higher than those in adjacent tissues of cancer (0.5cm) (17.113 ±3.367 vs 3.233 ± 1.898,P ＜0.05) and higher than those in adjacent tissues of cancer (1.0cm) (17.113 ± 3.367 vs 2.924 ± 1.612,P ＜ 0.05).However there were no significant differences in the expression levels of Survivin between adjacent tissues of cance (0.5cm) and adjacent tissues of cancer (1.0cm) (P ＞ 0.05).Besides the Pearson correlation analysis showed that the expression levels of PTEN protein were negatively correlated with those of Survivin protein in cancer tissues (r =-0.908,P ＜ 0.05).Conclusion PTEN and Survivin gene may jointly promote the pathogenesis and development of renal cancer,which has potential application and instruction value for gene-targeted therapy and surgery.