Experimental study on Geraniin in treatment of osteoporosis in ovariectomized mice
Abstract：Objective To observe the therapeutic effects of Geraniin on osteoporosis in ovariectomized mice,and to explore its possible action mechanism.Methods Eighteen female C57/BL6 mice were randomly divided into sham operation group,model group and treatment group,with 6 mice in each group.The mice in model group and treatment group were treated by surgery to exsect bilateral ovaries,however,the mice in sham operation group were treated by surgery to cut only little adipose tissues beside ovary and to reserve bilateral ovaries.Moreover the mice in treatment group were given Geraniin 20mg · kg-1 · d-1 on 5d before surgery,however,the mice in sham operation group were given equal dose of 0.9％ sodium chloride solution,with a treatment course of three months for the three groups.At the end of experiment,all the mice were sacrificed,and the serum levels of alkaline phosphatase (ALP),tartrate resistant acid phosphatase (TRAP),osteocalcin (BGP) and estradiol(E2) were detected.Furthermore the bone mineral density (BMD)detection and morphometric analysis were performed by micro CT.The fat cells count in bone marrow cavity was observed under light microscopy.Besides the key signaling molecules in Wnt/β-catenin signaling pathway including Wnt3a,β-catenin,GSK-3β,Axin2 and Runx2 were detected by Real time-PCR and Western Blot,respectively.Results As compared with those in sham operation group,the serum levels of ALP,TRAP and BGP were significantly increased in model group,however,the levels of E2 were decreased significantly,moreover the BMD,trabecular bone thickness,trabecular bone number,bone volume/total volume,the expression levels of Wnt3a,β-catenin,Axin2,Runx2 mRNA and proteins were significantly decreased,but the bone trabecular separation,fat cell count in bone marrow cavity as well as the expression levels of GSK-3β mRNA and proteins were increased significantly in model group (P ＜ 0.05).As compared with those in model group,the serum levels of ALP,TRAP and BGP were significantly decreased and the levels of E2 were increased significantly in treatment group,moreover,the BMD,trabecular bone thickness,trabecular bone number,bone volume/total volume as well as the expression levels of Wnt3a,β-catenin,Axin2,Runx2 mRNA and proteins were significandy increased,however the bone trabecular separation,fat cell count in bone marrow cavity,the expression levels of GSK-3β mRNA and protein were significantly decreased in treatment group (P ＜ 0.05).Conclusion The Geraniin has certain therapeutic effects on osteoporosis in ovariectomized mice,and its action mechanism may be related with the activation of Wnt/β-catenin signaling pathway.
Keywords：Geraniinovariectomized miceosteoporosisWnt/β-catenin signaling pathway
Publication Date：2017-01-01
Pages：4( 3685-3688 )