Mechanism of targeted silencing Survivin gene expression in human renal carcinoma cell line-786-O cells to reduce invasion ability of renal carcinoma in vitro

doi:10.3969/j.issn.1002-7386.2017.23.001

Abstract：Objective To investigate the mechanism of targeted silencing Survivin gene expression in human renal carcinoma cell line-786-O cells to reduce invasion ability of renal carcinoma .Methods The RNA inteference transfection technique was used to silence Survivin gene expression in human renal carcinoma 786-O cells.The expression levels of Survivin mRNA and protein as well as MMP-2 protein were detected by reverse transcription-polymerase chain reaction ( RT-PCR) and Western Blot,respectively.Results The 786-O/low-Survivin was established successfully .As compared with control siRNA/786-O and non-siRNA/786-O,and the Survivin siRNA/786-O showed obvious reduction of expression levels of Survivin mRNA and protein (P<0.01).Moreover the expression levels of MMP-2 protein were also down-regulated significantly in Survivin siRNA/786-O (P<0.01).However there were no significant differences in the expression levels of Survivin mRNA between siRNA/786-O control group and non-siRNA/786-O group (P>0.05).After Survivin siRNA was transfected into 786-O cells,which inhibited the transcription activity of Survivin gene .Western Blot assay showed that the expression levels of Survivin protein (2.424 ±0.303)in Survivin siRNA /786-O group were significantly lower than those (15.483 ±0.343) in siRNA/786-O control group as well as those (15.513 ±0.407) in non-siRNA/786-O group (P <0.01 ).Moreover the expression levels (0.484 ±0.111) of MMP-2 protein in Survivin siRNA/786-O group were significantly lower than those (10.861 ±1.347) in siRNA/786-O control group as well as those (10.947 ±1.744) in non-siRNA/786-O group (P<0.01).However there were no significant differences in the expression levels of Survivin protein and MMP -2 protein between siRNA/786-O control group and non-siRNA7/86 -O group (P>0.05).After Survivin siRNA was transfected into 786-O cells, which obviously down -regulated the expression levels of Survivin protein and MMP-2 protein.Conclusion The targeted silencing of Survivin gene can obviously down -regulate the expression levels of Survivin mRNA and protein in human renal carcinoma cell line -786-O cells,and can interfere the expression of MMP-2 protein,which suggests that Survivin gene may participate in the regulation mechanism of invasion and metastasis of renal cancer .