Effects of leptin by means of lateral ventricle injection on proopiomelanocortin in dorsal vagal complexin of rats

doi:10.3969/j.issn.1002-7386.2017.22.006

Abstract：Objective To investigate the effects of leptin by means of lateral ventricle injection on proopiomelanocortin in dorsal vagal complexin of rats ,and to explore whether the leptin can adjust proopiomelanocortin in dorsal vagal complex like hypothalamic arcuate nucleus ,so as to find a possible path that leptin plays a role in vagal complex .Methods Thirty-six SD rats were randomly divided into experimental group and control group ,with 18 rats in each group,moreover,the experimental group was redivided into 3 subgroups according to the death time after injection (1h,3h,5h),with 6 rats in each subgroup. The stainless steel catheter was detained in lateral ventricle of rats for 1 week, then the rats were free to resume and domesticated,then the rats in experimental group were given leptin 3.5μg/μl by lateral ventricle injection ,however,the rats in control group were given 0.9% sodium chloride solution 3.5μg/μl.Finally the dorsal vagal complex was located by Hematoxylin-eosin dyeing,and the expression of proopiomelanocortin was detected .Results On 1,3,5h after giving leptin by intracerebroventricular injection , there were no significant differences in the positive expression cell count and positive expression cell integral optical density of proopiomelanocortin in dorsal vagal complex between experimental group and control group (P>0.05).Moreover there were no significant differences in the positive expression cell count and positive expression cell integral optical density of proopiomelanocortin in dorsal vagal complex among the three subgroups (P>0.05).Conclusion Injecting leptin into lateral ventricle of rats can not result in the increase of proopiomelanocortin in dorsal vagal complex .