The apoptosis of non-small cell lung cancer A549 cells induced by PARP inhibitor-Olaparib in vitro through down-regulating the expression of SIRT1 gene
XU Qinghua
Abstract：Objective To investigate the effects of PARP inhibitor-Olaparib on the growth and apoptosis of non-small cell lung cancer A549 cells and its possible action mechanism .Methods The A549 cells were routinely cultured and treated with different doses of Olaparib (0、5,10,20,50μmol/L) and/or SRT1720(1μmol/L),EX527(1μmol/L)for 24h.Then MTT method was used to evaluate the proliferation of A 549 cells.Flow cytometer was used to analyze the apoptosis of A 549 cells, and Western Blot was performed to detect the expressions of SIRT 1 and apoptosis -related genes .Results After A549 cells were treated by different concentrations of Olaparib for 24h,the survival rate,the expression levels of SIRT1 and Bcl-2 of A549 cells were gradually decreased with the increase of Olaparib dosage ,meanwhile the apoptosis rate ,the expression levels of Bax and Caspase-3 of A549 cells were also gradually increased with the increase of Olaparib dosage ,with a dose-dependent manner (P<0.05).Moreover simple EX527 could simulate the effects of Olaparib on A549 cells (P <0.05),however, the SRT1720 combined with Olaparib could resist the effects of Olaparib as mentioned above .Conclusion PARP inhibitor-Olaparib can inhibit cell proliferation and induce apoptosis of A 549 cells by down-regulating the expression levels of SIRT 1 gene.
Keywords：non-small cell lung cancerPARP inhibitorOlaparibSIRT1apoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3374-3377 )