Expression of CCDC12 protein in colorectal cancer tissues and its relationship with chemotherapeutics resistance

doi:10.3969/j.issn.1002-7386.2017.20.005

Abstract：Objective To investigate the correlation and significance between expression of CCDC12 protein and therapeutic effects of chemotherapy on colorectal cancer. Methods The expressions levels of CCDC12, MRP-1, P-gp, Survivin, Apaf-1 proteins were detected by immunohistochemistry in 84 cases of colorectal cancer (tissue specimens). The inhibitory effects of chemotherapeutics 5-Fu, CDDP, L-OHP on tumor cells were detected by MTT assay. Moreover the expressions levels of CCDC12,MRP-1,P-gp,Survivin,Apaf-1 proteins in fresh tissues of 20 cases of colorectal cancer and mucous membrane tissues beside cancer were detected by Western Blot assay. Results The expressions levels of CCDC12, MRP-1,P-gp, Survivin proteins in colorectal cancer tissues were obviously higher than those in normal intestine mucous membrane,however, the expression levels of Apaf-1 were decreased in colorectal cancer tissues (P<0. 05). The inhibitory rates of 5-Fu, L-OHP on tumor cells with positive expression of CCDC12 were significantly lower than those with negative expression of CCDC12 (P<0. 05),but there was no obvious correlation between the inhibitory effects of CDDP on tumor cells and expression of CCDC12 (P >0. 05). The inhibition effects of 5-Fu on tumor cells were related with the expressions of MRP-1 and P-gp proteins, and the inhibition effects of CDDCP on tumor celles were related with expressions of Survivin protein,moreover, the inhibition effects of L-OHP were correlated to expressions of Survivin,Apaf-1 proteins. The correlation analysis showed that the expressions of CCDC12 protein in colorectal cancer tissues were positively correlated to those of P-gp, Survivin,however,which were negatively correlated to those of Apaf-1 (P <0. 05). Conclusion The expression levels of CCDC12 protein are increased in colorectal cancer tissues,which is correlated to the therapeutic effects of chemotherapy on colon cancer. Moreover CCDC12 may be involved in chemotherapeutics resistance,which may be a potential target protein for drug resistance reversion.