Study on oxidative damage markers in experimental rats with hypertriglyceridemia

doi:10.3969/j.issn.1002-7386.2017.20.003

Abstract：Objective To establish rat model with hypertriglyceridemia in order to observe the changes of oxidative damage markers and NADPH oxidase in experimental rats with hypertriglyceridemia. Methods Sixty male SD rats were randomly divided into control group ( n=20 ) , model group ( n=20 ) and positive drug group ( n=20 ) . The rats in model group and positive drug group were feed with high fat diet for 4 weeks. The serum levels of TG,HDL-C,LDL-C,TC,MDA, SOD,GSH-PX and TG,TC in liver at baseline and on 4 weeks after modeling were detected. The expression levels of of NOX1-mRNA and NOX4-mRNA in liver were measured by RT-PCR at the end of 6 weeks after modeling. The rats in positive drug group were given fenofibrate by gavage,100 mg·kg-1 ·d-1 for 2 weeks, and then the levels of TG,HDL-C,LDL-C and TC were detected. Results At the end of 6 weeks after modeling,the levels of TG,LDL-C,TC and TG,TC in model group and positive drug group were significantly increased,as compared with those at baseline (P<0. 05),however,the serum levels of HDL-C were obviously decreased,as compared with those at baseline (P<0. 05). Meanwhile,the serum levels of MDA in model group and positive drug group were significantly higher than those at baseline (P<0. 05),but the levels of SOD and GSH-PX were significantly lower than those at baseline(P < 0. 05). The expression levels of liver NOX1-mRNA and NOX4-mRNA at the end of 6 weeks after modeling in model group were significantly higher than those in control group and positive drug group (P<0. 05). On 2 weeks after treatment with fenofibrate,the serum levels of TG,TC and LDL-C in positive drug group were obviously decreased, however,the serum levels of HDL-C were significantly increased (P < 0. 05). Conclusion The rat models with hypertriglyceridemia are successfully established. The levels of GSH-PX and SOD in rats with hypertriglyceridemia are obviously decreased and the levels of MDA were significantly increased,and then NADPH oxidase is activated to induce oxidative damage in vivo.