Effects of PBMNC induced by IL-23 on the proliferation,apoptosis and gene expression of MUTZ-1 cells

doi:10.3969/j.issn.1002-7386.2017.18.004

Abstract：Objective To observe the effects of peripheral blood mononuclear cell (PBMNCs)induced by IL-23 on the proliferation,apoptosis and gene expression of MUTZ-1 cells-a human myelodysplastic syndrome (MDS) cell line. Methods The PBMNCs were isolated by ficoll density gradient centrifugation. The cells were divided into four groups:control group (without IL-23),three different concentration IL-23 groups (2,10,50ng/ ml),which were induced in vitro for 72 hours. Then PBMNCs were co-cultured with MUTZ-1 cells. MTT assay was used to detect the proliferation of MUTZ-1 cells. Flow cytometry was used to detect the cell cycle and cell apoptosis. Real time-PCR and Western-blot were used to detect the expression levels of Bax,Bcl-2,caspase-3 and survivin mRNA and protein. Results After MUTZ-1 cells were co-cultured with PBMNCs induced by 2,10,50ng/ ml IL-23,respectively,the cell proliferation rate of MUTZ-1 and cell proportion at phase S were significantly decreased,however,the cell apoptosis rate and cell proportion at phase G0 / G1 were significantly increased, with a concentration - dependent and time-dependent manner,as compared with those in control group (P < 0. 05 ). Meanwhile,the expression levels of Bax,caspase-3 mRNA and protein were obviously up-regulated,but the expression levels of Bcl-2,survivin mRNA and protein were significantly down-regulated,with a concentration-dependent and time-dependent manner,as compared with those in control group (P < 0. 05). Conclusion The PBMNCs induced by IL-23 can inhibit cell proliferation of MUTZ-1 cells and can promote apoptosis of MUTZ-1 cell,and its action mechanism may be correlated with up-regulating the expressions of Bax and caspase-3 and down-regulating the expressions of Bcl-2 and survivin.