Clinical significance of miR-185 detection in gastric cancer tissues and its relationship with Vav3-Rac1 pathway

doi:10.3969/j.issn.1002-7386.2017.17.001

Abstract：Objective To investigate the expression and relationship of miR-185,Vav3,Rac1 gene in gastric cancer tissues and adjacent normal tissues.Methods The expression levels of miR-185,Vav3,Rac1 mRNA were detected by fluorescence real time quantitative polymerase chain reaction (QPCR) assay in 100 cases of gastric cancer tissues and 80 cases of adjacent normal tissues.The expression differences of miR-185,Vav3,Rac1 were compared between gastric cancer tissues and adjacent normal tissues.The ROC curve was completed with data,and the correlation between the expressions of miR-185, Vav3,Rac1 mRNA and biological characteristics of patients was analyzed.Moreover the correlation among miR-185,Vav3,Rac1 in gastric cancer tissues was analyzed.Results The expression levels of miR-185 in gastric cancer tissues were obviously lower than those in adjacent normal tissues,however,the expression levels of Vav3,Rac1 mRNA were significantly higher than those in adjacent normal tissues (P<0.05).The optimized cut-off value of miR-185,Vav3,Rac1 under ROC curve was 0.3440,0.3346,0.2944,respectively.The expression of miR-185 was closely correlated to depth of invasion,differentiation extent and lymphatic metastasis of gastric cancer,moreover, the expressions of Vav3 and Rac1 mRNA were closely related with differentiation extent and lymphatic metastasis of gastric cancer (P<0.05).Spearman correlation analysis showed that there was an negative correlation between miR-185 and Vav3,miR-185 and there was a positive correlation between Vav3 and Rac1 (P<0.05).Conclusion The expression levels of miR-185 are obviously decreased in gastric cancer tissues,moreover,which are closely correlated to biological characteristics of gastric cancer,and miR-185 may be involved in progression of gastric cancer by regulating Vav3-Rac1 pathway.