Expression and significance of SSBP1 in hepatocellular carcinoma tissues

MA Rongfen; CHEN Qiuying; LIU Yanfen

doi:10.3969/j.issn.1002-7386.2017.16.006

Abstract：Objective To investigate the expression and significance of mitochondria single-strand DNA protein 1 (SSBP1) in hepatocellular carcinoma tissues.Methods The primary hepatocellular carcinoma tissue samples from 80 patients who were admitted and treated by surgical ablation in our hospital from December 2013 to December 2016 were collected.The expression levels of SSBP1 mRNA and protein in 80 cases of hepatocellular carcinoma tissues and cancer adjacent tissues were detected by Real time-PCR and immunohistochemistry (SP).The correlation between SSBP1 expression levels and clinicopathological parameters of hepatocellular carcinoma was analyzed.moreover the expression levels of SSBP1 in hepatocellular carcinoma cell strains including HepG2, MHCC97H, SMMC7721,Huh7 as well as normal hepatocellular line-L-027 were detected.Results The expression levels and positive expression rate of SSBP1 in cancer tissues and adjacent tissues were 1.87±0.23,0.36±0.05 and 80.00% (64/80),15.00% (12/80), respectively,and the differences were statistically significant (P<0.05).The expression levels and positive expression rate of SSBP1 in cancer tissues were obviously higher than those in cancer adjacent tissues (P<0.05).Moreover the expression levels and positive expression rate of SSBP1 in HepG2 cells were significantly higher than those in MHCC97H,SMMC7721,Huh7 and L-027 cells (P<0.05).Furthermore the expression levels of SSBP1 were closely correlated to tumor size, AFP,TNM clinical stage,differentiation degree,portal vein tumor thrombus and lymph node metastasis (P<0.05).Conclusion The overexpression of SSBP1 exists in hepatocellular carcinoma tissues and cell strains,which is related with clinicopathological parameters of hepatocellular carcinoma,moreover, the high-expression of SSBP1 is closely correlated to poor prognosis of patients.