The unique characteristics of multidrug resistant Acinetobacter baumannii biofilm revealed by difference gene analysis
Abstract：Objective To investigate the unique gene expression of multidrug resistnat Acinetobacter baumannii biofilm,and to expore its acton mechanism.Methods The multidrug resistant Acinetobacter baumannii strains were selected, and the cells were grown in two different status including floatings thallus at rapid growth phase and thw thallus that growed on biofilm.The two samples were used to extract mRNA and analyze the whole gene expression,and to compare the differences of gene expression.Finally the materialization of expression difference gene in gene function was analyzed.Results In the two different states of the same strain,the two genes which growed at rapid growth phase on biofilm were all extracted and analyzed.The expression of the two genes was different.The biofilm status was indeed a unique way of existence,with its own unique biological behavior and gene expression.In comparison between the two samples,that the expressions of 106 genes were up-regulated,however, the expressions of the other 92 genes were down-regulated.Conclusion The proteins encoded by expression differences genes are involved in biofilm formation and morphous maintenance,which are regulated by specific signaling pathways,signaling factors and transcription factor.In floatings bacteria status,they were not expressed or were not obviously expressed.
Keywords：multidrug resistance Acinetobacter baumanniigenetic analysisbiofilmhigh-flux sequencing
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 2414-2418,2422 )