Effects of down-regulation of gene expression of 14-3-3σ on proliferation of renal cancer cells
QIU Wei
SHEN Yongqing
ZHANG Aili
Abstract：Objective To investigate the expressions of 14-3-3σ in renal cell carcinoma tissues ( RCC ) and in normal renal tissues ( NT ) , and to explore the role of 14-3-3σ and its action mechanism in RCC. Methods Forty-seven patient with renal cancer who enderwent radical nephrectomy were enrolled in the study. The RNA and protein were extracted from RCC tissues and normal renal tissues,and the expression levels of 14-3-3σwere detected by RT-PCR and Western blot, respectively. The changes of cell proliferation were detected by CCK-8 assays in 786-O cells after overexpression or knockdown of gene expressions of 14-3-3σ. Results As compared with those in normal renal tissues, the expression levels of 14-3-3σmRNA and protein in renal cancer tissues were significantly decreased (P<0. 01),moreover, the overexpression of 14-3-3σgene could significantly inhibit the proliferation of 786-O cells (P < 0. 05),however, the knockdown of 14-3-3σ gene expression could increase the cell proliferation (P < 0. 01). Conclusion The expression levels of 14-3-3σ gene are significantly down-regulated in RCC tissues,which may be correlated to the the proliferation of renal cancer cells.
Keywords：renal carcinoma14-3-3σproliferationRT-PCR
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 2085-2089 )