Effects of extract of ginkgo biloba leaf on liver fibrosis of rats and its action mechanism against liver fibrosis

SHANG Rui; WU Jun; ZHENG Xuejiao

doi:10.3969/j.issn.1002-7386.2017.13.002

Abstract：Objective To investigate the effects of extract of ginkgo biloba leaf (GBE) on liver fibrosis of rats,and to explore its action mechanism against liver fibrosis.Methods Sixty SD rats were randomly divided into normal control group (n =12) and model group (n =48).The rats in model group were redivided into four subgroups:model control group,low-dose GBE group,median-dose GBE group and high-dose GBE group,with 12 rats in each group.The rats in three GBE groups were given GBE 50mg/kg,100mg/kg,200mg/kg,respectively by gavage,however,the rats in normal control group and model control group were given equal volume of 0.9% sodium chloride solution,once a day for 2 months.After administration,the serum levels of ALT,AST,MDA,SOD,albumin,globulin (albumin/globulin) were detected by Kit.The liver fibrosis indexes including HA,PC-Ⅲ,C-Ⅳ and LN were measured by radioimmunoassay.After the rats were sacrificed,the right lobe of the liver tissue was taken out,and the expressin levels of TGF-β1,TIMP1,typeⅠcollagen mRNA were detected by RT-PCR.Results As compared with those in model control group,the serum levels of AST,ALT,globulin and hepatic fibrosis indexes in median-dose GBE group and high-dose GBE group were significantly decreased,and the expression levels of TGF-β1,TIMP1 and typeⅠcollagen mRNA were significantly decreased too (P<0.05).However the levels of albumin and the ratio of albumin/globulus were significantly increased (P<0.05),but the levels of MDA were not changed obviously.Conclusion The extract of ginkgo biloba leaf can significantly improve liver function indexes and hepatic fibrosis indexes of rats with liver fibrosis,and its action may be completed by reducing the expression level of TGF-β1,TIMP1 and type Ⅰcollagen mRNA.