Experimental study on the effects of nedaplatin on expression levels of nasopharyngeal carcinoma related proteins-P53,Bcl-2,caspase
LI Yan
LI Rong
Abstract：Objective To observe the effects of nedaplatin on cell proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cells (NPC CNE-2),and to explore the correlation between nedaplatin and the expression levels of nasopharyngeal carcinoma related proteins-P53,Bcl-2,caspase.Methods The effects of nedaplatin on cell proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cells in different concentrations (0μg/ml,5μg/ml,10μg/ml,20μg/ml) and in different action time points (12h,24h,36h,48h) were detected by MTT and flow cytometry,respectively.Moreover the effects of nedaplatin on expression levels of P53,Bcl-2,caspase of CNE-2 cells in different concentrations were detected by immunohistochemisty.Results MTT examination results showed that with increase of concentrations of nedaplatin,OD values of CNE-2 cell proliferation were gradually decreased (P<0.05),moreover,with the prolongation of action time of nedaplatin,the OD values were gradually decreased (P<0.05).In concentrations of nedaplatin 0μg/ml,the CNE-2 cell proliferation was faster,cells were full and cell morphous was regular,however, with increase of concentrations of nedaplatin, cell proliferation was gradually decreased,cell became round,with edge unsharpness.The flow cytometry detection results showed that with increase of concentrations of nedaplatin,the apoptotic rate of CNE-2 cells was gradually increased (P<0.05).The immunohistochemisty results showed that with increase of concentrations of nedaplatin, expression levels of P53,Bcl-2 were gradually decreased (P<0.05),however,the expression levels of caspase were gradually increased (P<0.05).Conclusion The nedaplatin can obviously inhibit the cell proliferation and promote cell apoptosis of NPC CNE-2 cells,which may be correlated to its effects of inhibiting the expressions of p53 and bcl-2 protein and promoting the expression of caspase protein.
Keywords：nedaplatinnasopharyngeal carcinoma CNE-2 cellscell proliferationcell apoptosisP53Bcl-2caspase
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1285-1288 )