Effects of culture supernatant of ovarian cancer cell line-SKOV3 on costimulatory molecules of macrophages SONG Yuxia
Abstract：Objective To investigate the changes of subtypes of macrophage treated by culture supernatant of ovarian cancer cell line-SKOV3 in vitro,and to explore the differences of expressions of CD80,CD86,CD40 in different subtypes of macrophage.Methods The peripheral blood mononuclear cells were separated from healthy adults by Ficoll density gradient method,which were induced into macrophages by GM-CSF.Then the macrophages were cultured in supernatant of SKOV3 ovarian cancer cells at exponential growth phase for 14 days.The changes of subtypes of macrophage and the expression levels of CD80,CD86,CD40 in different subtypes of macrophages were detected by flow cytometry.Results The examination results by flow cytometry showed that after the macrophages were treated in supernatant of SKOV3 ovarian cancer cells for 14 days, the macrophages were mainly differentiated into CD163-M2 subtype macrophages [M1/M2=(12.37±1.76)%/(22.23±2.21)%].The expression levels of CD40 in CD163-M2 subtype macrophages were significantly decreased,as compared with those in CD64-M1 subtype macrophage ( P <0.05).However there were no significant differences in the expression levels of CD80 and CD86 between two groups ( P >0.05).Conclusion After the macrophages are treated in culture supernatant of SKOV3 ovarian cancer cells,which are differentiated into different subtypes.Moreover the expression levels of costimulatory molecules are down-regulated in M2 subtype macrophages,as compared with those in M1 subtype macrophages ,which suggests that these costimulatory molecules play an important role in activation of macrophages and immune response,which may be correlated to immune escape of tumor cells.
Keywords：ovarian carcinomaM2 subtype macrophagesCD80/CD86/CD40
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1144-1147 )