Protective effect and action mechanism of water extract of gentian on liver damage of rats with acute pancreatitis

WU Jun; SHANG Rui

doi:10.3969/j.issn.1002-7386.2017.08.005

Abstract：Objective To investigate the protective effect and action mechanism of water extract of gentian on liver damage of rats with acute pancreatitis (AP).Methods Forty SD rats were randomly divided into normal control group ( n =8) and model group ( n =32).The animal models with AP were established by injecting 5% cow-bezoar sodium cholate through the duodeno papillary retrograde cholangiopancreatic duct.After modeling,the rats were redivided into four subgroups :model control group,low-dose group (water extract of gentian 12mg/kg),median-dose group (24mg/kg) and high-dose group (48mg/kg),with 8 rats in each group.The rats in low-dose,median-dose and high-dose groups were given water extract of gentiane 12mg/kg,24 mg/kg,48 mg/kg,respectively by gavage,however,the rats in normal control group and model control group were given equal volume of 0.9% sodium chloride solution,once a day for 1 week.After administration,the serum levels of ALT, AST, AMY, IL-6 and TNF-α were detected.Moreover the rats were sacrificed and liver specimens were taken out,the changes of liver structure were observed by HE staining,and the expression levels of NF-κB and I-κB in liver tissue were detected by Western Blot.Results As compared with those in model control group, the serum levels of ALT,AST,AMY, IL-6,TNF-α in median-dose group and high-dose group were significantly decreased ( P <0.01),and the expression levels of NF-κβprotein were obviously decreased too ( P <0.01),however, the expression levels of I-κB protein were significantly increased ( P <0.01).Conclusion The water extract of gentian can obviously improve the liver damage caused by acute pancreatitis in rats.