Expression and clinical significance of miR-15a in endometrial carcinoma
SHAO Tian
Abstract：Objective To investigate the expression and clinical significance of miR-15a in endometrial carcinoma tissues in order to explore the possible action mechanism of miR-15a in the pathogenesis and development of endometrial carcinoma.Methods The expression levels of of miR-15a were detected by fluorescence quantitative PCR in 68 cases of endometrial carcinoma tissues and 68 cases of adjacent tissues of tumor.The independent sequence analog (non-transfection group),miR-15a analog (transfection group) was transfected into RL95-2 endometrial cancer cells,respectively, moreover, an negative control group was established (control group).The expression levels of miR-15a and matrix metalloproteinase2 (MMP-2) were detected and compared among the three groups.Besides the invasion ability and migration ability of RL95-2 cells were detected by Transwell tumor cell invasion assay and Transwell tumor cell migration assay.Results The relative expression levels of miR-15a in endometrial carcinoma tissues were significantly lower than those in adjacent tissues of tumor (P<0.05).The relative expression levels of miR-15a were correlated to FIGO staging,lymph node metastasis, muscular layer infiltration degree and differentiation degree of endometrial carcinoma ( P <0.05).The relative expression levels of miR-15a in transfection group were significantly higher than those in non-transfection group and control group,however the relative expression levels of MMP-2 in transfection group were significantly lower than those in non-transfection group and control group ( P <0.05).Besides the invasion ability and migration ability of RL95-2 cells in transfection group were significantly lower than those in non-transfection group and control group ( P <0.05).Conclusion The expression levels of miR-15a in patients with endometrial carcinoma are lower,moreover,which are correlated with lymph node metastasis,differentiation degree,myometrial invasion degree,FIGO staging.As a tumor suppressor gene, miR-15a may play an inhibitory role in the metastasis and invasion of cancer cells by inhibiting the expression of MMP-2 protein.
Keywords：endometrial carcinomamiR-15aexpression levelsmetastasis and invasionanalysis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1133-1136 )