The expression of PTEN/PI3K/VEGF signaling pathway in arch artery of animal models with Kawasaki disease

doi:10.3969/j.issn.1002-7386.2017.08.002

Abstract：Objective To observe the changes of VEGF/PTEN/PI3K signal pathway in young rabbit model with Kawasaki disease,and to explore its possible mechanism.Methods Forty young rabbits were randomly divided into control group ( n =10) and model group ( n =30),The rabbits in model group were intravenously injected with 10% bovine serum albumin at 1d and 14d (2.5ml/kg) to establish the animal models with Kawasaki disease,however,the rabbits in control group were given the same volume 0.9% sodium chloride solution by intravenous injection.The specimens of serum and arch artery were collected at 1d,7d and 30 days after modeling.the serm levels of WBC,CK and VEG were measured,and the morphological changes of arch artery were observed by HE staining,moreover, the expression levels of PTEN and PI3K in arch arteries were detected by immunohistochemical staining.Results As compared with those in control group,on the first days after modeling,the serum levels of VGEF in model group were significantly increased,which indicated that there was an inflammatory reaction in the body of rabbits,however, on the 7th day after modeling, the serum levels of VEGF were obviously decreased ( P <0.01),until the 30th day after modeling,VEGF levels were recovered to normal level.Immunohistochemical results showed that on the first day after modeling,the expression levels of PTEN in arch artery were increased, moreover, on the 7th day and 30th day after modeling,the expression levels of PTEN were significantly increased (P<0.01),however,the expression levels of PI3K were gradually decreased ( P <0.01).Conclusion The VEGF/PTEN/PI3K signaling pathway is closely related to the pathogenesis and development of Kawasaki disease.