Effectso f Huoxuexiaoyi recipe on endometrial receptivity in mcie models with endometriosis

doi:10.3969/j.issn.1002-7386.2017.07.001

Abstract：Objective To investigate the effects of Huoxuexiaoyi recipe on endometrial receptivity in mice models with endometriosis , and to explore its action mechanism .Methods The animal models with endometriosis pregnancy hypofunction were established in mice through the method of abdominal cavity +subcutaneous injections , and the mice in sham-operation group were injected with same volume of 0.9% sodium chloride solution .After the success of modeling, the mice were randomly divided into traditional Chinese medicine group ( TCM group) and model group .The mice in TCM group were treated by Huoxuexiaoyi recipe ,however ,the mice in model group and sham-operation group were given same volume of 0.5% CMC by gavage.After three menstrual cycles, the mice in the three groups were put in the cage with infertile male mice (2:1), then the vaginal plug was observed at 8:00 am in the next day (D1).The detection specimens were taken at 10:30 pm on D4 after false pregnant ,and the expression levels of endometrial integrin-αvβ3 and leukaemia inhibitory factor ( LIF) were detected by Western Blot .Results The expression levels of endometrial integrin-αvβ3 and LIF at implantation window phase in model group were significantly decreased ,as compared with those in sham-operation group ( P <0.05).However the expression levels of endometrial integrin-αvβ3 and LIF at implantation window phase in TCM group were significantly increased,as compared with those in model group ( P <0.05 ), but which were significantly lower than those in sham-operation group ( P <0.05 ).Conclusion The endometriosis has adverse effects on endometrial receptivity . Huoxuexiaoyi recipe can significantly increase the expression levels of endometrial integrin αvβ3 and LIF at implantation window phase so as to improve endometrial receptivity in mice with endometriosis .