Expression and clinical significance of Notch1 and P16 in chondrosarcoma

WANG Kemeng; WEI Shiping

doi:10.3969/j.issn.1002-7386.2017.06.005

Abstract：Objective To observe the expressions of Notch1 protein and P16 protein in chondrosarcoma in order to explore their action mechanism in the pathogenesis and and development of chondrosarcoma.Methods The expression levels of Notch1 protein and P16 protein were detected by immunohistochemistry (SP) in 46 cases of chondrosarcoma,18 cases of osteochondroma and 10 cases of normal cartilage tissues.Results The positive expression rate of Notch1 protein in osteochondroma and chondrosarcoma was significantly higher than that in normal cartilage tissues (P ＜ 0.05),moreover,the positive expression rates of Notch1 protein in chondrosarcoma was significantly higher than that in osteochondroma (P ＜0.05).The positive expression rate of Notch1 protein was gradually increased in different histological grades of chondrosarcoma (grade Ⅰ,grade Ⅱ,grade Ⅲ),with the decrease of differentiation degree (P ＜ 0.05).The pdsitive expressions of P16 protein were observed in normal cartilage tissues and in osteochondroma tissues.The positive expression rate of P16 protein in chondrosarcoma was 39.1％ (18/46),which was significantly lower than that in osteochondroma tissues and in normal cartilage tissues (P ＜ 0.05).The positive expression rate of P16 protein in different histological grades of chondrosarcoma (grade Ⅰ,grade Ⅱ,grade Ⅲ) was 68.8％ (11/16),30％ (6/20),20％ (1/10),respectively,which was gradually decreased with the decrease of differentiation degree (P ＜ 0.05).The expressions of Notch1 protein and P16 protein were not correlated to patient's age,sex,tumor size,tumor position (P ＞ 0.05).Conclusion The high-expression of Notch1 protein and low-expression of P16 protein may synergistically promote the proliferation ability of chondrosarcomatous malignant cells,which may play an important role in the pathogenesis and development of chondrosarcoma,and the research results provide pathological basis for clinical treatment and further research.