Expression and clinical significance of TGF-β1 and LMO1 in gastric cancer tissues

doi:10.3969/j.issn.1002-7386.2017.06.004

Abstract：Objective To observe the expression of transforming growth factor beta 1 (TGF-β1) and LMO1 in gastric cancer tissues,and to explore their roles in the pathogenesis and development of gastric cancer.Methods The expression levels of TGF-β1 and LMO1 mRNA and protein in 60 cases of gastric carcinoma tissues and 50 cases of normal gastric mucosa tissues were detected by Real time-PCR and Western Blot,moreover,the expression levels of TGF-β1 and LMO1 protein were detected by immunohistochemistry (SP).Besides the correlation between expressions of TGF-β1,LMO1 and clinical pathological characteristics was analyzed.Results The expression levels of TGF-β1 and LMO1 mRNA in gastric carcinoma tissues were significantly higher than those in normal gastric mucosa tissues (P ＜ 0.05).Moreover the positive expression rates of TGF-β1 and LMO1 proteins in gastric cancer tissues were 85.0％ (51/60),83.33％ (50/60),respectively,which were significantly higher than those in normal gastric mucosa tissues [12.0％ (6/50),8.0％ (4/50),respectively,P ＜0.05].The expressions of TGF-β1 and LMO1 were not correlated to patient's age,sex,tumor position (P ＞ 0.05),however,which were correlated with infiltration depth of tumor,differentiation degree of tumor,TNM staging and lymph node metastasis (P ＜ 0.05).Moreover the expression of TGF-β1 was positively correlated to that of LMO1 in gastric carcinoma (r =0.875,P ＜0.01).Conclusion The overexpressions of TGF-β1 and LMO1 exist in gastric carcinoma tissues,which may play some roles in the pathogenesis and development of gastric cancer.Therefor TGF-β1 and LMO1 can be regarded as one of molecule indexes to evaluate the malignant biological behavior of gastric cancer and may become potential targets for the treatment of gastric cancer.