Intervention effects of CGRP on renal interstitial fibrosis and expression of inflammatory cytokines in mice with unilateral ureteral obstruction
Abstract：Objective To investigate the effects of calcitonin gene-related peptide ( CGRP ) on renal interstitial fibrosis and expression of Inflammatory cytokines in mice with unilateral ureteral obstruction (UUO).Methods The animal models with UUO were established in 36 mice.The mice in treatment group were given CGRP 1.0nmol · kg-1 · d-1 by intravenous injection,however,the mice in model group were given equivalent 0.9% sodium chloride solution by intravenous injection.The mice in both groups were sacrificed after 14-day administration,then the percentage of renal tubular lesion ,the extent of renal interstitial fibrosis , number of renal interstitial macrophage cells , the expression levels of renal interstitial expression of α-smooth muscle actin (α-SMA), monocyte chemotactic protein-1 (MCP-1) and F4/80 mRNA were observed and compared between two groups .Results CGRP alleviated significantly renal tubular lesion and renal interstitial fibrosis (P<0.05 ).The expression levels of α-SMA and macrophages number in renal interstitial in treatment groups were significantly lower than those in model group ( P <0.05).Moreover CGRP inhibited significantly the expression levels of MCP-1 and F4/80 mRNA ( P <0.05).Conclusion CGRP can reduce macrophage infiltration of mice with UUO ,decrease the expression levels of MCP-1 and F4/80 mRNA,inhibit the expression of α-SMA so as to relieve renal interstitial fibrosis .
Keywords：calcitonin gene-related peptideunilateral ureteral obstruction modelrenal interstitial fibrosismacrophages
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 540-544 )