Changes of expressions of TRPV1 and its sensitizing factor in serum, eutopic endometrium and ectopic endometrium of patients with endometriosis

doi:10.3969/j.issn.1002-7386.2017.04.009

Abstract：Objective To investigate the change regulation of expressions of TRPV 1 and its sensitizing factor in serum, eutopic endometrium and ectopic endometrium of patients with endometriosis in order to explore the pathogenesis mechanism of endometriosis .Methods Seventy patients with endometriosis who were admitted and treated in our hospital from October 2013 to October 2015 were randomly divided into dysmenorrhea group ( n =45) and non-pain group ( n =25). Besides the other 30 patients with uterine fibroids who underwent synchronization surgery were served as control group . Moreover the 45 patients in dysmenorrhea group were redivided into 3 subgroups by means of Visual Analogue Scale ,including mild,medium,severe dysmenorrhea groups,with 15 patients in each group.The expression levels of of TRPV1 and sensitizing factors including NGF, BKB1R, PGF2αin serum, eutopic endometrium and ectopic endometrium of patients with endometriosis were detected by ELISA ,immunohistochemistry and Western Blot ,respectively .Results The expression levels of NGF,BK and PGF2αin dysmenorrhea group were significantly higher than those in control group and non -pain group ( P<0.05).The expression of NGF in serum was positively correlated to that of PGF 2α,and the expression of BK was positively correlated to that of PGF2α( P <0.05).The Pearson correlation analysis showed that expressions of NGF ,BK, PGF2αwere positively correlated with VAS ( P <0.05).The results by immunohistochemistry and Western Blot showed that the expression levels of NGF,BKB1R and TRPV1 in dysmenorrhea group were significantly higher than those in non -pain group and control group ( P <0.05).The correlation analysis showed that the expression of NGF was positively correlated to that of TRPVl , BKB1R,TRPV1( P <0.05).Conclusion TRPV1 and its sensitizing factor including NGF ,BKB1R,PGF2αmay play an important role in the pathogenesis mechanism of dysmenorrhea in patients with endometriosis .