Expression of PKM2 in non-small cell lung cancer and its correlation with radiotherapy sensitivity

doi:10.3969/j.issn.1002-7386.2017.04.006

Abstract：Objective To investigate the expression of PKM 2 in non-small cell lung cancer and its correlation with radiotherapy sensitivity .Methods Sixty-eight patients with non -small cell lung cancer who were treated in our hospital from July 2013 to July 2014 were enrolled in the study .All the patients were diagnosed by clinical and operative pathology and were treated by radiotherapy .According to radiotherapy sensitivity , these patients were divided into group A ( sensitivity to radiotherapy ) and group B ( non -sensitivity to radiotherapy ) .The expression levels of PKM 2 in surgical ablation tumor tissues before radiotherapy were detected by immunohistochemistry , the expression levels of PKM 2 mRNA were detected by RT-PCR and the expression levels of PKM 2 protein were detected by Western Blot .Results The immunohistochemistry examination showed that the high expression rate of PKM2 in group A and group B was 21.62%(8/37)and 58.06%(18/31), respectively,there was a significant difference between two groups ( P <0.05).The results by RT-PCR showed that the expression levels of PKM2 mRNA in group B were significantly increased ,as compared with those in group A ( P <0.05). Western Blotting revealed that the expression levels of PKM 2 protein in group A were obviously decreased ,as compared with those in group A ( P <0.01).Conclusion The expression of PKM2 is negatively correlated with radiotherapy sensitivity , which shows that the expression of PKM 2 can be regarded as an important molecular mark to predict radiotherapy sensitivity in patients with non-small cell lung cance so as to realize individualized treatment .