Study on the protective effects and possible mechanism of erythropoietin and carbamoylation on cardiomyocytes apoptosis of rats with chronic heart failure

doi:10.3969/j.issn.1002-7386.2017.04.001

Abstract：Objective To investigate the protective effects and possible mechanism of erythropoietin ( EPO ) and carbamoylation on cardiomyocytes apoptosis of rats with chronic heart failure (CHF).Methods Thirty-nine healthy male SD rats were randomly divided into control group , EPO group and CEPO group .The animal models with heart failure were established by intraperitoneal injection with ISO-epinephrine in all the rats .After 4 -week intervention , the changes of hemodynamic parameters,the serum levels of iNOS and SOD as well as apoptotic index (AI) and apoptosis rate of myocardial cells were detected and calculated .Results As compared with those in control group , LVSP and ±dp/dtmax in EPO group and CEPO group were significantly increased ,however, HR and LVEDP were significantly decreased ( P <0.05).There were significant differences in hemodynamic parameters between EPO group and CEPO group ( P <0.05).As compared with those in control group, SOD levels in EPO group and CEPO group were significantly increased ( P <0.05),however, iNOS levels were significantly decreased ( P <0.05).There were significant differences in iNOS and SOD levels between EPO group and CEPO group ( P <0.05).Among the three groups,the AI in control group was the highest (36.53%),the next in order EPO group (237.3 %),which in CEPO group was the lowest (19.64%),there was a significant difference in AI among the three groups ( P <0.05).Moreover the decrease order of apoptosis rate in different time points was control group ,EPO group, CEPO ( P <0.05).Conclusion The EPO and CEPO can effectively improve the cardiac function of rats with CHF ,and the inhibition efficiency of CEPO is superior to that of CPO ,and its action mechanism may be related to the changes of serum levels of iNOS and SOD .