Effects of puerarin combined with telmisartan on Toll-like receptor/nuclear factor-κB inflammatory signal pathway in patients with obesity hypertension

doi:10.3969/j.issn.1002-7386.2017.03.011

Abstract：Objective To investigate the effect of puerarin combined with telmisartan on Toll-like receptor/nuclear factor-κB ( TLR/NF-κB) inflammatory signal pathway in patients with obesity hypertension .Methods A total of 160 patients with obesity hypertension who were admitted and treated in our hospital from June 2013 to June 2015 were enrolled as case group,and the other 100 healthy subjects were served as normal control group .The expression levels of TLR2 ,TLR4 mRNA in vascular endothelial cells were detected by Real time-PCR.In addition, the patients in case group were randomly redivided into observation group ( n =80) and control group ( n =80).The patients in control group were treated by telmisartan ,however, the patients in observation group ,on the basis of control group , were given puerarin ,with a treatment course of one month for both groups.After treatment, The expression levels of TLR2, TLR4, NF-κB and IκBa at mRNA and protein were detected by Real time-PCR and Western Blot , respectively .Furthermore the serum levels of TNF-α, IL-6 and hs-CRP were detected by ELISA.Results As compared with those in normal control group ,the expression levels of TLR2 and TLR4 mRNA in case group were significantly increased ( P <0.05).After treatment ,the expression levels of TLR2, TLR4, NF-κB mRNA and protein and the serum levels of TNF-α, IL-6, hs-CRP in control group and observation group were significantly decreased , however , the expression levels of IκBa mRNA and protein in both groups were significantly increased ,as compared with those before treatment ( P <0.05),but the improvement degree of these parameters in observation group was superior to that in control group ( P <0.05).Conclusion The obvious immune inflammatory reaction disorder exists in patients with obesity hypertension .Puerarin combined with telmisartan can obviously down -regulate the activation of TLR/NF-κB inflammatory signal pathway and release of inflammatory factors so us to perform protective effects on vascular endothelial injury .