Effect of RNA interference targeting at LOXL2 gene on the invasion of gastric cancer cell line-BGC823

doi:10.3969/j.issn.1002-7386.2017.02.004

Abstract：Objective To observe the inhibition effects of small interference RNA ( siRNA) on the expression of LOXL2 in gastric cancer cells ,and to explore the effects and action mechanism of LOXL 2 gene on the invasion ability of human gastric cancer cell line-BGC823 .Methods The expression levels of LOXL 2 in gastric cancer tissues , adjacent tissues and human gastric cancer cell line-BGC823, gastric epithelial cell line-GES-1 were detected by Western Blot .Moreover LOXL2-siRNA was synthesized and tansfected into BGC 823 cells.The invasion ability of BGC823 cells was detected by Transwells assay.Besides the expression levels of MMP-2,MMP-9 were detected by qPCR and Western Blot before and after transfection.Results The expression levels of LOXL2 in BGC823 cells were significantly higher than those in GES-1 cells (P<0.01).The expression levels of LOXL2 mRNA and protein in BGC823 cells in LOXL2-siRNA group were significantly decreased,as compared with those in non -transfection group and negative plasmid control group ( P <0.05).The invasion experiment showed that the cell counts of breaking cell membrane in BGC 823 cells of LOXL2-siRNA group were significantly less than those in non -transfection group and negative plasmid control group ( P <0.05) .Moreover the expression levels of MMP-2,MMP-9 in BGC823 cells after transfection were significantly decreased ,as compared with those before transfection (P<0.05).Conclusion To inhibit the expression of LOXL2 in BGC823 cells can obviously decrease the invasion ability of BGC823 cells.