Effects of naringenin on the expressions of osteoclast specific genes and OPG/RANKL/RANK in osteoclasts

doi:10.3969/j.issn.1002-7386.2017.02.001

Abstract：Objective To observe the effects of naringenin on the expressions osteoclast specific genes -CATK,MMP-9,TRAP and OPG/RANKL/RANK in osteoclasts.Methods The osteoclasts were cultured by whole bone marrow cells induction method.The experiment was divided into 5 groups:blank control group,HG-DMEM induction group,HG-DMEM+naringenin (0.1μmol/L) group,HG-DMEM +naringenin (1μmol/L) group and HG-DMEM +naringenin (10μmol/L) group.After HE and TRAP stainings , the cell morphous was observed under inverted microscope .The TRAP positive cell number was detected by spectrophotometer .The expression levels of CATK , MMP-9,TRAP as well as OPG/RANKL/RANK mRNA and protein were detected by Real time-PCR and Western Blot .Results As compared with those in blank control group, the TRAP positive cell number and the expression levels of TRAP ,MMP-9,CATK,RANKL,RANK mRNA and protein were significantly increased in HG-DMEM induction group , however , the expression levels of OPG mRNA and protein were significantly decreased ( P <0.05).As compared with HG-DMEM induction group, after 24-hour pretreatment, naringenin could obviously decrease TRAP positive cell number and the expression levels of CATK , MMP9-, TRAP, RANKL, RANK mRNA and protein ,and could increase the expression levels of OPG mRNA and protein ,with a dose-dependent way ( P <0 .05 ) .Conclusion Naringenin can inhibit the generation and differentiation of osteoclasts ,and the action may be realized by regulating OPG/RANKL/RANK signal transduction pathway to inhibit the expressions of TRAP , MMP-9,CATK.