Effects of demethylation drugs combined with paclitaxel on invasion ability of renal carcinoma cells and their action mechanism

doi:10.3969/j.issn.1002-7386.2016.24.009

Abstract：Objective To observe whether combination application of deoxidation cytidine ( DAC) with paclitaxel ( PTX) has a synergistic effect on renal carcinoma cells , and to explore their action mechanism .Methods The renal clear cell carcinoma-ACHN cell line was cultured in vitro .The 120 culture dishes were randomly divided into 4 groups:blank control group,DAC group,PTX group,combination group (DAC+PTX),with 30 dishes in each group.The cells in DAC group were treated by DAC 0.5, 1, 2, 4, 8μmol/L, respectively for 3 days;the celles in PTX group were treated by PTX 1, 2, 4, 8, 16 nmol/L, respectively for 3 days; the cells in combination group were treated by DAC +PTX for 3 days.The apoptosis rates of renal carcinoma cells induced by T peptide combined with dendritic cells were detected by flow cytometry , and the expression levels of lymphocyte enhancement factor (LEF1),E-cadherin and Caspass-3 in renal carcinoma cells were detected by ELISA .Results The inhibitory effects on tumor cells in DAC group , PTX group and combination group were obvious,in which the inhibitory effects in combination group were the most obvious ( P <0.05).Moreover the inhibitory effects on tumor cells in DAC group and PTX group were in direct proportion to the drug concentrations ( P <0.05).As compared with those in DAC group and in PTX group , the expression levels of LEF 1 in combination group were the lowest (P<0.05), however, the expression levels of E-cadherin and Caspass-3 were the highest.Moreover the inhibitory effects of DAC and PTX on the expressions of LEF 1 as well as the promotive effects on the expressions of E-cadherin ,Caspass-3 were in opposite proportion to the drug concentrations ( P <0.05).Conclusion The combination intervention of DAC and PTX on renal carcinoma cells has a synergistic effect .Moreover LEF1 is an new therapeutic target for renal carcinoma .The combination application of DAC with PTX can increase the apoptosis rate of renal carcinoma cells , and can decrease the abilities of invasion and metastasis of renal carcinoma cells .