Effects of isoliquiritin on the radiotherapy sensitization in nude mice models of transplantation tumor with nasopharyngeal carcinoma of CNE2 cells
GUO Junyu
CHEN Yingchao
Abstract：Objective To observe the radiotherapy sensitization of isoliquiritin in nude mice models of transplantation tumor with nasopharyngeal carcinoma of CNE 2 cells,and to explore the correlation between isoliquiritin and the expression levels of HIF-1αand VEGF.Methods The nasopharygeal cell line -CNE2 cells were inoculated into 20 female nude mice, when tumor nodule reached 4 ~6mm,these mice were randomly divided into four groups:control group, isoliquiritin group (50mg? kg-1? w-1 ),radiotherapy group (20Gy), isoliquiritin (50mg? kg-1? w-1 ) combined radiotherapy (20Gy) group (combination treatment group).The tumor size was measured regularly .After 20 days,the mice were sacrificed,the volume and weight of tumors were detected , moreover , the tumor inhibition rates were calculated and the expression levels of HIF-1αand VEGF mRNA were detected by Real -time PCR in the four groups .Results The volume and weight of tumors in combination treatment group were significantly decreased ,as compared with those in the other three groups ( P <0.05). Moreover the expression levels of IF-1αand VEGF mRNA in combination treatment group were significantly decreased , as compared with those in control group and radiotherapy group ( P <0.05 ).Conclusion Isoliquiritin can enhance radiotherapy sensitization in nude mice of transplantation tumor with nasopharyngeal carcinoma ,and its action mechanism may be correlated to its inhibitory effects on expressions of HIF-1αand VEGF so us to inhibit tumor angiogenesis .
Keywords：isoliquiritinnasopharyngeal neoplasmsradiotherapy sensitizationHIF-1αVEGF
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3704-3707 )