Clinical significance of detection of EGFR and ALK genes by CT guided puncture biopsy

HU Xiaofei; WANG Jun; WANG Gangsheng

doi:10.3969/j.issn.1002-7386.2016.24.004

Abstract：Objective To observe the detection rate and positive rate of epidermal growth factor receptor ( EGFR) and anaplastic lymphoma kinase ( ALK) gene by lung cancer puncture biopsy under CT guidance , and to analyze its clinical application value .Methods Two hundred and eighty specimens from the patients with lung cancer who were treated in our hospital from January 2013 to February 2015 were taken by CT guided puncture biopsy ,which were divided into two groups:observation group ( n =200,thick needle puncture) and control group ( n =80,thin needle puncture).The clinicopathological characteristics, gene mutation correlation,positive rate, detection rate,tumor cell number and postoperative complications were observed and compared between two groups .Results The gene mutation rate of EGFR in female patients was 66.04%, however, which was 28.74%in male patients,there was a significant difference between two groups ( P <0.05).Moreover there was a significant difference in gene mutation rate of EGFR between non-smoking patients and smoking patients (57.24%vs 29.63%, P <0.05).However there were no significant differences in patien ’s age,pathological staging,gene mutation rate of EGFR between two groups ( P >0.05 ).The gene mutation rate of EGFR in patients with lung adenocarcinoma was significantly higher than that in the patients with the other types of lung carcinomas ( P <0.05).There were no significant differences in the expression levels of ALK among different sex and age , pathological differentiation degree ( P >0.05), in which the gene mutation rate of ALK in patients at stage Ⅳwas significantly higher than that in the patients at the other stages ( P <0.05).The detection rate and positive rate of EGFR ,ALK in observation group were significantly higher than those in control group ( P <0.05).The average tumor cell numbers detected in observation group were much more than those in control group ( P <0.05).Besides the total incidence rates of postoperative complications in observation group were significantly higher than those in control group ( P <0.05).Conclusion The detection rates of EGFR ,ALK gene by thick needle puncture biopsy are higher than those by thin needle puncture biopsy , however, thin needle puncture biopsy has still certain clinical significance , moreover,the incidence rates of postoperative complications in thin needle puncture biopsy are lower than those in thick needle puncture biopsy ,thus, both detection methods have certain clinical diagnostic value .