GC-MS analysis for the volatile constituents of Lasiosphaera calvatia extracted by different extraction methods
Abstract：Objective To use GC-MS to analyze the volatile constituents of Lasiosphaera calvatia obtained by different extraction methods in order to provide scientific basis for evaluating and controlling the quality of Lasiosphaera calvatia.Methods 〗The volatile oil constituents in Lasiosphaera calvatia were extracted by steam distillation method and ethyl acetate ultrasound extraction method ,respectively ,then the volatile oil constituents in Lasiosphaera calvatia were analyzed and identified by means of GC-MS.Moreover the two kinds of extraction methods were compared .Results The volatile oil compositions extracted by steam distillation method were mainly phenol ,2,2'-methylenebis[6-(1,1-dimethylethy)]-4-methyl and 7-amino-1,4-dimethylpyrimido [4,5-c] pyridazine-3,5-(1H,2H)-dionethe,however, which extracted by ethyl acetate ultrasound extraction method were mainly hexadecanoic acid ethyl ester ,moreover, which extracted by ethyl ether extraction method were mainly the common composition of 2,2'-methylene double (4-methyl-6-tertiary butyl phenol ).Conclusion There are significant differences in the volatile constituents of Lasiosphaera calvatia extracted by different extraction methods , thus it is need to normalize and standardize the quality control of Lasiosphaera calvatia further .The research results in this article provide a certain reference and basis for quality control of puffballs .
Keywords：Lasiosphaera calvatiaGC-MSvolatile constituents
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3545-3547 )