Clinical significance of expressions of miR-381, PCNA, Cyclin D1, p21 in gastric cancer tissues

doi:10.3969/j.issn.1002-7386.2016.23.004

Abstract：Objective To analyze the clinical significance of expressions of microRNA-381 ( miR-381 ) , PCNA, Cyclin D1,p21 in human gastric cancer tissues and adjacent normal tissues ,and to explore their correlation .Methods Real time fluorescent quantify RT-PCR (qRT-PCR) was used to detect the expression levels of miR-381,PCNA,Cyclin D1,p21 in 85 cases of gastric cancer tissues and 50 cases of para -carcinoma normal tissues .The correlation between miR-381and clinicopathological characteristics of patients and the correlation between miR-381 and PCNA,Cyclin D1,p21 were analyzed. Results The expression levels of miR-381 in gastric cancer tissues were significantly lower than those in adjacent normal tissues ( P <0.05),however, the expression levels of PCNA,Cyclin D1 mRNA in gastric cancer tissues were significantly higher than those in adjacent normal tissues ( P <0.05),but the expression levels of p21 mRNA in gastric cancer tissues were significantly lower than those in adjacent normal tissues ( P <0.05).The expressions of miR-381 were closely correlated to the differentiation and lymphatic metastasis of gastric cancer ( P <0.05), however, which were not related with the other clinicopathological characteristics of gastric cancer ( P >0.05).The correlation analysis showed that the expression of miR-381 was negatively correlated to that of Cyclin D1 ( r =-0.3864, P <0.05),however,which was positively correlated to that of p21 ( r =0.3223, P <0.05).Conclusion The expression of miR-381 is related with clinicopathological characteristics of gastric cancers , and miR-381 may be involved in the development of gastric cancer by regulating the expressions of Cyclin D1 and p21.