Detection of influenza virus A and B by modified multiplex real-time fluorescence PCR assay
Abstract：Objective To set up a modified multiplex real-time fluorescence quantitative PCR ( MRT-PCR) assay to detect influenza virus A and B rapidly .Methods The MRT-PCR assay was established according to target sequence of influenza virus A and B specific gene selected by bioinformatics analysis results .The standard products and quality control samples were built to evaluate the sensitivity , specificity and repeatability of the system .Results The MRT-PCR detection system was successfully established based on Taqman-molecular beacon ( Taqman-MB) probe and Homo-Taq Assidted Non-Dimer ( HAND) system.The detection limit of the assay was 102 copies/μl,which showed good specificity and repeatability , with coefficient of variation (CV) being 0.99%~2.50%.Conclusion A fast,specific,sensitive,stable, modified MRT-PCR detection system is successfully established ,which possesses favourable application prospect in clinical practice .
Keywords：MRT-PCRHAND systemTaqman-molecular beaconinfluenza virus
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3529-3532 )