Effects of baicalin on the expression levels of interleukin-8 in gingival tissues and peripheral blood of rats with periodontitis

doi:10.3969/j.issn.1002-7386.2016.220.02

Abstract：Objective To observe the effects of baicalin on the expression levels of interleukin-8 in gingival tissues and peripheral blood of rats with periodontitis induced by colibacillus lipopolysaccharide (LPS).Methods Fifty SD rats (8-week age ) were randomly divided into five groups, with 10 rats in each group, which were control group ( group A ) , periodontitis group (group B),low-dose baicalin treatment group (0.01μg/ml,group C1),median-dose baicalin treatment group (0.1μg/ml,group C2),high-dose baicalin treatment group (1.0μg/ml,group C3).After 1-week treatment, these rats were sacrificed,and the specimens of gingival tissues and peripheral blood were obtained,then the expression levels of IL-8 in peripheral blood of rats were measured by cytometric bead array ( CBA) ,and the expression levels of IL-8 in gingival tissues of rats were detected by ELISA.Results The expression levels of IL-8 in peripheral blood in group B were significantly higher than those in control group ( P <0.05).The expression levels of IL-8 in gingival tissues and peripheral blood in the three baicalin treatment groups were significantly higher than those in group B ( P <0.05) ,moreover, the expression levels of IL-8 in group C1, C2, C3 were gradually decreased in order ( P <0.05).Conclusion The baicalin can decrease the expression levels of IL-8 in gingival tissues and peripheral blood of rats with periodontitis and can inhibit the destructive effects of LPS on periodontal tissues of rats.