Significance of the expression of small RNA-21 and the changes of high-risk HPV viral load in the pathogenesis and development of cervical cancer

WANG Bin

doi:10.3969/j.issn.1002-7386.2016.20.004

Abstract：Objective To investigate the significance of the expression of small RNA-21 (miR-21) and the changes of high-risk HPV viral load in the pathogenesis and development of cervical cancer.Methods One hundred and fifty patients with cervical lesion who were admitted and treated in our hospital from March 2011 to March 2015 were enrolled in the study, and all the patients underwent cervical biopsy.These patients were divided into CINⅠgroup (n=39),CINⅡ group ( n =37),CINⅢgroup ( n =34), CC group ( n =40).The expression levels of miR-21 and HR-HPV load in different types of cervical lesions were detected.The correlation between the expressins of miR-21 and HR-HPV load was analyzed,moreover, the correlation between the expressins of miR-21 as well as HR-HPV load and clinicopathological charateristics of cervical cancer was analyzed.Results The expression levels of miR-21 in patients with poorly differentiated tumor cells ( 6.12 ± 0.83) were significantly higher than those (8.17 ±0.65) in patients with moderately differentiated tumor cells and those (11.54 ±1.29) in patients with well differentiated tumor cells ( P <0.05).The HR-HPV load (26.53 ±3.12) in patients with tumor diameter >4cm was significantly higher than that (23.60 ±2.85) of patients with tumor diameter <4cm ( P <0.05).The HR-HPV load (23.86 ±2.34) in patients at FIGO staging Ⅰ~ⅡA was obviously lower than that (27.59 ± 3.12) in patients at FIGO stagingⅡB~Ⅳ.The expression levels of miR-21 in HR-HPV-positive patients (9.75 ±0.83) were significantly higher than those (5.47 ±0.46) in HR-HPV-negative patients ( P <0.05).However there were no significant differences in the expression levels of miR-21, HR-HPV load, patient's age, FIGO staging, pathological types, lymphatic metastasis and vessel infiltration ( P >0.05).The percentage (41.03%) of HR-HPV -positive patients (41.03%) in CINⅠgroup (41.03%) was significantly lower that that in CINⅡ group (67.57%), CIN Ⅲ group (85.29%), CC group (92.50%), moreover, the relative expression levels of mi-R21 in CINⅠgroup (6.42 ±0.53) was significantly lower than that in CINⅡ group (7.26 ±0.61),CIN Ⅲ group (8.24 ±0.65) and CC group (9.36 ±0.70) (P<0.05).Conclusion The expression levels of miR-21 can reflect the differentiation degree of cervical cancer cells,and high-risk HPV viral load can reflect FIGO stages of cervical cancer and diameter range of cancer,moreover,the expression of miR-21 is positively correlated to high risk HPV viral load.The combined detection of miR-21 with high risk viral load can suggest cervical precancerous lesions, the pathogenesis and development of cervical cancer.