St udy on the synthesis of Tf-USPIO nanometer particle and overexpressoi n of TfR tran sgenic neural stem celsl
Abstract：Objective To synthesize Tf-USPIO nanometer particle and establish the neural stem cells system that can express transferrin receptors ( TfR) stably.Methods The neural stem cells were transfected by lentivirus,then the neural stem cells that could express TfR stably were selected out by flow cytometry.The expression levels of TfR were detected by Western Blotting.The TF was coupled with USPIO to synthesize Tf-USPIO nanometer particle,which were measured by DLS method,with the diameters of USPIO and Tf-USPIO being (36 ±0.7) nm,(43.5 ±0.08) nm, respectively.Results The neural stem cells were transfected by lentivirus successfully, and the neural stem cells that could express TfR stably were selected out by flow cytometry successfully,moreover, the overexpression of TfR was identified by Western Blotting.The stable Tf-USPIO nanometer particles were synthesized by link-coupled method.Conclusion The TfR neural stem cells line is successfully established,which can synthesize stable Tf-USPIO nanometer particles.
Keywords：ultra-small superparamagnetic iron oxidetransferrin receptorslentivirus vectorstem cellmagnetic resonance imaging
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3045-3048 )