Effects of ligustrazine on ethological changes in rats with acute spinal cord injury

doi:10.3969/j.issn.1002-7386.2016.18.013

Abstract：Objective To observe the effects of ligustrazine on ethological changes in rats with acute spinal cord injury.Methods Seventy healthy adult SD rats were randomly divided into control group ( n =10), spinal cord injury group (SCI group, n =30) and drug treatment group ( n =30).The rats in SCI group and drug treatment group were divided into 1-day, 3-day and 14-day subgroups after treated with different measures.The morphological changes in spinal cord of rats on1d,3d,14d after operation were detected by HE staining and electron microscopy,respectively, at the same time, the motor function in hindlimb of rats were evaluated by Jacobs classification criterion and BBB scoring .Results HE staining results showed that the number of normal motor neuron in drug treatment group was more than that in SCI group.The electron microscopy results showed that the degree of pycnosis of nerve cells,staining degree of chromatin and the vacuolization of hyalomitome in drug treatment group were obviously relieved,as compared with those in SCI group.Moreover ,the paraplegia rate on 14d after treatment in drug treatment group was 40%,which was significantly lower than that (70%) in SCI group, besides the BBB scores in drug treatment group on 14d after treatment were significantly higher than those in SCI group (10.6 ±2.7 vs 7.9 ±2.0, P <0.05).Conclusion The ligustrazine can improve ethological changes of rats after acute spinal cord injury and has certain therapeutic effects on spinal cord injury in rats.