Effects of Xiaoaiping injection combined with mitomycin on lung cancer cells in vitro
XIE Zexin
YANG Yang
WANG Jinshuo
Abstract：Objective To observe the effects of Xiaoaiping injection combined with mitomycin on cell proliferation, cell cycle, cell apoptosis and expressions of related genes (CyclinD1,P53 mRNA) of lung cancer-A549 cells in vitro,and to explore potential action mechanism.Methods The inhibitory effects of Xiaoaiping injection (2,8,21μl/ml) combined with mitomycin (2μg/nl) were detected by MTT (combination treatment group A,B,C),moreover, control group, Xiaoaiping group and mitomycin group were established.The cell cycle and cell apoptosis of lung cancer-A549 cells in vitro were detected by cytometry,and the expression levels of CyclinD1 and P53 mRNA were measured by RT-PCR.Results The results by MTT showed that the optical density value (OD) on 24h,48h,72h in combination treatment group A,B,C was significantly higher than that in mitomycin group ( P <0.05).The results by cytometry showed that as compared with control group,the lung cancer cells were blocked at G0/G1 phase in Xiaoaiping group and mitomycin group ( P <0.05).There were significant differences in the cell ratio at G0 /G1 phase and cell apoptosis rate between combination treatment groups and mitomycin group ( P <0.05).The expression levels of P53 mRNA in Xiaoaiping group and mitomycin group were significantly increased,as compared with those in control group ( P <0.05).Moreover the expression levels of P53 mRNA in combination treatment groups were significantly higher than those in mitomycin group ( P <0.05).The expression levels of Cyclin D1 in Xiaoaiping group and mitomycin group were significantly decreased,as compared with those in control group ( P <0.05).Furthermore the expression levels of Cyclin D1 in combination treatment groups were significantly decreased,as compared with those in mitomycin group ( P <0.05).Conclusion Xiaoaiping injection combined with mitomycin can enhance the efficiency of chemotherapy on lung cancer-A549 cells in vitro,and the action mechanism may be correlated to up -regulating the expression levels of P53 mRNA,increasing cell apoptosis and down -regulating the expressions of CyclinD1 mRNA to block cancer cells at G0/G1 phase.
Keywords：Xiaoaiping injectionmitomycincell cycleapoptosisCyclinD1P5
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2737-2740 )