Experimental study on the effects of exogenetic estrogen on the ability of human periodontal ligament stem cell osteogenic differentiation
PAN Feng
DING Yin
LI Baoyong
Abstract：Objective To investigate the effects of exogenetic estrogen on the differentiation ability of human periodontal ligament stem cell ( PDLSCs) in vitro. Methods The PDLSCs were cultured in non-phenol red osteogenesis induction medium with different concentrations of 17β-E2 ( E10 -7 , E10 -8 , E10 -9 ) , moreover, which were cultured in osteogenesis induction medium+0. 01% dehydrated alcohol (control group), besides,-which were cultured in the medium with different concentrations of estradiol ( E10 -7 , E10 -8 , E10 -9 ) and were cultured in osteogenesis induction medium +ICI182780 (ICI group), and osteogenesis induction medium+ 10 -7β-E2+10 -7ICI182780 (10 -7 +ICI group),respectively. The cell shape, cell proliferation activity,alkaline phosphatase ( ALP) activity,the synthesis ability of typeⅠcollagen were detected in every group. Results After treated by exogenetic estrogen,the cell proliferation was speeded up,cell growth was intensived,with spiral arrangement. As compared with that in control group, the cell proliferation on 3 days,5 days in estrogen intervention group was obviously inhibited ( P <0. 05),moreover, the cell proliferation in estrogen intervention group was higher than that in control group ( P <0. 05). Among difeerent drug concentration groups, the effects in E10 -7 group on cell proliferation were the most obvious, and the cell proliferation levels in E10 -7 group were significantly higher than those in E10 -8 group and E10 -9 group ( P <0. 05). The espression of ALP showed dynamic changes, the levels of ALP from the third day in all the groups were increased,however,which in estrogen intervention group were significantly higher than those in control group ( P <0. 05). Among different drug concentration groups, the levels of ALP in E10 -7 group were significantly higher than those in E10 -8 group and in E10 -9 group ( P <0. 05). However there were no significant differences between ICI group and E10 -7 +ICI group ( P >0. 05). As compared with that in control group,the typeⅠcollagen synthesis of PDLSCs had an enhancement trend, moreover, with a drug dose-dependent way. Conclusion The estrogen can promote osteogenic differentiation process in PDLSCs,moreover, its action is closely correlated to estrogen concentration.
Keywords：stem cellhuman periodontal ligament stem cellestrogenosteogenic differentiation
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 2611-2614,2618 )