Effects of naringenin on osteoporosis in ovariectomized rats

doi:10.3969/j.issn.1002-7386.2016.17.004

Abstract：Objective To evaluate the therapeutic effects of naringenin on osteoporosis in ovariectomized rats. Methods Fifty female SD rats were randomly divided into sham-operation group, model group, low-dose naringenin group, median-dose naringenin group and high-dose naringenin group, with 10 rats in each group. Except for the rats in sham-operation group, the rats in the other 4 groups underwent ovariectomy. On 4 weeks after operation, the rats in low-dose naringenin group,median-dose naringenin group and high-dose naringenin group were given naringenin by gavage, with the dosage of 35mg/kg, 70mg/kg, 140mg/kg, respectively for 12 weeks. Then the serum levels of estradiol (E2), progesterone (P), tartrate resistant acid phosphatase (TRACP), bone alkaline phosphatase (ALP), osteocalcin (BGP), urine calcium (Ca), phosphorus (P) and deoxypyridinoline (DPD), bone mineral density (BMD), the maximum bending stress,loading force of the 5th lumbar vertebra and morphologic changes of bone trabecula were detected and compared among the 5 groups. Results As compared with that in sham-operation group,the body weight in model group was obviously increased, however, uterus organ coefficient was significantly decreased ( P <0. 05). The body weight in the three medication groups was obviously decreased, however, uterus organ coefficient was significantly increased,as compared with that in model group ( P<0. 05),with a dose-dependent manner. As compared with those in sham-operation group,the serum levels of E2 and P in model group were significantly decreased ( P <0. 05). However the serum levels of E2 and P in the three medication groups were significantly increased, as compared with those in model group ( P <0. 05),with a dose-dependent manner. As compared with those in sham-operation group,the serum levels of TRACP,Ca,P,DPD in model group were significantly increased ( P <0. 05),however, which in the three medication groups were significantly decreased,as compared with that in model group (P<0. 05), with a dose-dependent manner. As compared with those in sham-operation group,the serum levels of ALP in model group were obviously increased, however, the seum levels of BGP were significantly decreased ( P <0. 05). The serum levels of ALP in the three medication groups were significantly decreased,however,the serum levels of BGP were significantly increased,as compared with those in model group ( P <0. 05),with a dose-dependent manner. As compared with that in sham-operation group,the BMD was significantly decreased in model group ( P <0. 05). The levels of BMD in the three medication groups were significantly increased, as compared with those in model group ( P <0. 05), with a dose-dependent manner. As compared with those in sham-operation group,the maximum bending stress and loading force of the 5th lumbar vertebra in model group were significantly decreased ( P <0. 05). The maximum bending stress and loading force of the 5th lumbar vertebra in low-dose,median-dose and high-dose naringenin groups were significantly increased, as compared with those in model group ( P <0. 05),with a dose-dependent manner. As compared with those in sham-operation group,the percentage of bone trabecula area, bone trabecula number and thickness of bone trabecula were significantly increased in model group ( P <0. 05). The percentage of bone trabecula area, bone trabecula number and thickness of bone trabecula in the three medication groups were significantly increased, as compared with those in model group ( P <0. 05),however the resolution of bone trabecula area in the three medication groups was significantly decreased ( P <0. 05), with a dose-dependent manner. Conclusion Naringenin has better therapeutic effects on ovariectomy-induced osteoporosis in rats.